monoclonal antibody for chlamydia spp  (Meridian Life Science)

Journal: European Journal of Microbiology & Immunology

Article Title: Borrelia and Chlamydia Can Form Mixed Biofilms in Infected Human Skin Tissues

doi: 10.1556/1886.2019.00003

Figure Lengend Snippet: (A) A multiple sequence alignment obtained from Clustal Omega analyses representing BL Chlamydia OmpA DNA sequence mapped against different Chlamydia strains of Chlamydia psittaci (KM247620), Chlamydia trachomatis (EU040365), and Chlamydia pneumoniae (KC512913). (B) Clustel Omega multiple sequence alignment of OmpA gene DNA sequences obtained from BL tissues against different Chlamydia strains such as Chlamydia trachomatis (JX559522), Chlamydia psittaci (HM214490), and Chlamydia pneumoniae (DQ358972). Asterisks represent identical nucleotide sequence in all four Chlamydia sequences

Article Snippet: The slides were then treated with a dilution of 1:200 (dilution buffer: PBS pH 7.4 + 0.5% BSA) of monoclonal antibody for Chlamydia spp. (Cat# C65815M, Meridian Life Sciences, USA) and incubated overnight in a humidified chamber at 4 °C.

Techniques: Sequencing

Journal: European Journal of Microbiology & Immunology

Article Title: Borrelia and Chlamydia Can Form Mixed Biofilms in Infected Human Skin Tissues

doi: 10.1556/1886.2019.00003

Figure Lengend Snippet: Representative IHC images of Borrelia, Chlamydia , and alginate staining in Borrelia -infected BL skin tissues. Panels A, F, K, P, and V show IHC staining results of skin tissues using a FITC labeled anti -Borrelia antibody (green arrows and arrowheads). Panels B, G, L, Q, and W show staining results with anti-chlamydia antibody (red arrows). Panels C, H, M, R, and X show staining of anti-alginate antibody (blue arrows). Panels D, I, N, S, and Y show results of staining with non-specific IgG antibody. Panels E, J, O, T, and Z are the differential interference contrast (DIC) images that show the morphology of the tissues. Panels A–O corresponds to BL skin tissues while panels P–T include negative controls corresponding to skin tissues from healthy human foreskin, and panels V–Z include negative controls corresponding to healthy skin tissues. All images were taken at 200× magnification. Scale bar: 200 μm

Article Snippet: The slides were then treated with a dilution of 1:200 (dilution buffer: PBS pH 7.4 + 0.5% BSA) of monoclonal antibody for Chlamydia spp. (Cat# C65815M, Meridian Life Sciences, USA) and incubated overnight in a humidified chamber at 4 °C.

Techniques: Staining, Infection, Immunohistochemistry, Labeling

Journal: European Journal of Microbiology & Immunology

Article Title: Borrelia and Chlamydia Can Form Mixed Biofilms in Infected Human Skin Tissues

doi: 10.1556/1886.2019.00003

Figure Lengend Snippet: Representative images of IHC staining of BL biopsy skin tissues with Borrelia, Chlamydia , and alginate-specific antibodies. Panels A, E, I, M, and R show IHC positive staining for Borrelia (green arrows), and panels B and F show positive staining of Chlamydia (red arrows), while panels J, N, and S show negative staining for Chlamydia spp. Panel C, G, K, O, and T depict positive staining for alginate (blue arrows). Panel D, H, L, P, and V show DIC images. All images were taken at 400× magnification. Scale Bar: 200 μm

Article Snippet: The slides were then treated with a dilution of 1:200 (dilution buffer: PBS pH 7.4 + 0.5% BSA) of monoclonal antibody for Chlamydia spp. (Cat# C65815M, Meridian Life Sciences, USA) and incubated overnight in a humidified chamber at 4 °C.

Techniques: Immunohistochemistry, Staining, Negative Staining

Journal: European Journal of Microbiology & Immunology

Article Title: Borrelia and Chlamydia Can Form Mixed Biofilms in Infected Human Skin Tissues

doi: 10.1556/1886.2019.00003

Figure Lengend Snippet: Quantitative analysis of Borrelia biofilms for positive Borrelia and Chlamydia IHC staining

Article Snippet: The slides were then treated with a dilution of 1:200 (dilution buffer: PBS pH 7.4 + 0.5% BSA) of monoclonal antibody for Chlamydia spp. (Cat# C65815M, Meridian Life Sciences, USA) and incubated overnight in a humidified chamber at 4 °C.

Techniques: Immunohistochemistry

Journal: European Journal of Microbiology & Immunology

Article Title: Borrelia and Chlamydia Can Form Mixed Biofilms in Infected Human Skin Tissues

doi: 10.1556/1886.2019.00003

Figure Lengend Snippet: Representative images of the IHC staining on BL skin tissue section and the images of the consecutive slides stained with combined fluorescent in situ hybridization (FISH) and IHC techniques. Panels A, B, and C are the results of skin tissues stained with antibodies against Borrelia (green arrow), Chlamydia (red arrow), and alginate (blue arrow), respectively. Panels E and I show the staining results of skin tissues with 16S rDNA probe for Borrelia burgdorferi (green arrow). Panels F and J show the staining results of skin tissues with 16S rDNA probe for Chlamydia spp. (red arrow). Panel G is stained with antibodies for alginate (blue arrow). Panels D and H depict the morphology of the skin tissues using DIC microscopy methods. As comprehensive negative controls, a competing oligonucleotide (panels I and J for Borrelia and Chlamydia , respectively), a random DNA probe (panel K), and a DNase-treated samples (panel L) were used on consecutive tissue sections to further show the specific city of the 16S rDNA probe (for further details of the experimental conditions can be in Materials and Methods). All images were taken at 400× magnification. Scale bar: 100 μm

Article Snippet: The slides were then treated with a dilution of 1:200 (dilution buffer: PBS pH 7.4 + 0.5% BSA) of monoclonal antibody for Chlamydia spp. (Cat# C65815M, Meridian Life Sciences, USA) and incubated overnight in a humidified chamber at 4 °C.

Techniques: Immunohistochemistry, Staining, In Situ Hybridization, Microscopy

Journal: European Journal of Microbiology & Immunology

Article Title: Borrelia and Chlamydia Can Form Mixed Biofilms in Infected Human Skin Tissues

doi: 10.1556/1886.2019.00003

Figure Lengend Snippet: Three-dimensional (3D) analyses of Borrelia and Chlamydia mixed biofilm in human BL skin biopsy tissue using confocal microscopy. Panels A, B, and C are the results of skin tissues positively immunostained with antibodies against Borrelia (green arrow), Chlamydia (red arrow), and alginate (blue arrow), respectively. Panel D shows the DIC image to depict the morphology of the tissue. Confocal microscopy shows the 3D distribution of mixed biofilms and the individual Z stacks focus on the biofilms showing Borrelia and Chlamydia (panel F) and Borrelia and alginate (panel G) spatial distribution. Scale bar: 100 μm

Article Snippet: The slides were then treated with a dilution of 1:200 (dilution buffer: PBS pH 7.4 + 0.5% BSA) of monoclonal antibody for Chlamydia spp. (Cat# C65815M, Meridian Life Sciences, USA) and incubated overnight in a humidified chamber at 4 °C.

Techniques: Confocal Microscopy